驴 anti-绵羊 IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed
Rockland 713-708-168
FACS, FM
Polyclonal
IgG
PE
F(ab')2 fragment
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- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- Heavy & Light Chain
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适用
- 绵羊
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宿主
- 驴
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克隆类型
- 多克隆
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标记
- PE
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应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- Supplier Product No.
- 713-708-168
- Supplier
- Rockland
- 原理
- F(ab')2 Sheep IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against Ch GP Ham Hs Hu Ms Rb & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG Fc, or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Rat Serum Proteins.
- 产品特性
- Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
- 免疫原
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Immunogen: Anti-Sheep IgG was produced by repeated immunization with sheep IgG whole molecule in donkey.
Immunogen Type: Native Protein
- 亚型
- IgG
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Donkey anti-Sheep IgG antibody (DyLight 680) - Preadsorbed
WB, FLISA, FM Polyclonal IgG DyLight 680
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- 应用备注
- Application Note: Antibody Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Optimal titers for other applications should be determined by the researcher. Flow Cytometry Dilution: 1:100 - 1:250 IF Microscopy Dilution: 1:100 - 1:250 Other: FLOW 1:100 - 1:250
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 0.5 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated antibody generated in donkey detects specifically sheep IgG. This secondary phycoerythrin conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.
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