山羊 anti-人 IgM (Fc5mu Region) Antibody (Alkaline Phosphatase (AP))
Quick Overview for 山羊 anti-人 IgM (Fc5mu Region) Antibody (Alkaline Phosphatase (AP)) (ABIN237630)
抗原
克隆类型
标记
应用范围
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抗原表位
- Fc5mu Region
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适用
- 人
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宿主
- 山羊
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交叉反应 （详细）
- Based on immunoelectrophoresis, the antibody reacts with the Fc5u portion of the human IgM heavy chain. No antibody was detected against normal human IgG or IgA, or against non-immunoglobulin serum proteins, but the antibody may cross-react with IgM from other species.
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产品特性
- Goat anti Human IgM (Fcu), Goat antibody to Human Immunoglobulin M (IgM), Fc5u specific Alkaline Phosphatase conjugated
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纯化方法
- Isolated from antisera by Immuno Affinity Chromatography using antigens coupled to agarose beads.
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Mouse anti-Human IgM antibody (HRP)
ELISA, WB Monoclonal CH2 IgG1 kappa HRP
Goat anti-Human IgM antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Rabbit anti-Human IgM antibodyELISA, IHC, WB Polyclonal unconjugated
Goat anti-Human IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Mouse anti-Human IgM antibody (SPRD)ELISA, FACS Monoclonal UHB IgG3 SPRD
Goat anti-Human IgM antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgM antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human IgM antibody (FITC)ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Human IgM (Heavy Chain) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
Goat anti-Human IgM (Heavy Chain) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
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应用备注
- A dilution range of 1:5.000 to 1:50.000 is suggested for ELISA and Western blotting. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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说明
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Concentration (detection method): 0.6 mg/mL (prior to lyophilization)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute to 1 mL with distilled water.
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缓冲液
- Lyophilized from 0.01 M Tris-HCl, 0.21 M Sodium chloride, pH 8.0 containing 15 mg/mL BSA, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
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注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution only prior to immediate use. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. -
储存条件
- 4 °C
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储存方法
- Store lyophilized product at 2-8 °C. After reconstitution, product is stable for several weeks at 2-8 °C as an undiluted liquid. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol (ACS or better grade) to make a final glycerol concentration of 50 % followed by storage at -28 °C with or without aliquoting.
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有效期
- 12 months
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- IgM
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物质类
- Antibody
抗原
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