山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (DyLight 488)
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北京 101111
Quick Overview for 山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (DyLight 488) (ABIN1990978)
抗原
片段
克隆类型
标记
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 山羊
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特异性
- Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulins. No reactivity is observed to: non-immunoglobulin mouse serum proteins, IgG from human or mouse
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交叉反应 （详细）
- min. cross-reactivity to human, mouse lgG
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产品特性
- DyLight® 488 has a maximum absorbance at 493 nm, Emax = 518 nm.
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纯化方法
- affinity purified goat IgG
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免疫原
- Purified Rat IgG, whole molecule
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Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 488) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG Atto 488
Goat anti-Rat IgG (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Mouse IgG antibody (HRP) - PreadsorbedELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment
Goat anti-Rat IgG antibody (DyLight 488)WB, FLISA, FM Polyclonal IgG DyLight 488
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG Atto 647N
Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rat IgG antibody (Cy3) - PreadsorbedWB, FACS, FLISA, FM, DB Polyclonal IgG Cy3
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应用备注
- 1:20 - 1:2,000 for most applications
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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缓冲液
- Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
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注意事项
- Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
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储存条件
- 4 °C/-20 °C
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储存方法
- Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- IgG
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物质类
- Antibody
抗原
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