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小鼠 anti-人 IgG (Fc Region) Antibody

ELISA Monoclonal 27B11E9 IgG1 kappa unconjugated
产品编号 ABIN7477947
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
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    Fc Region
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    应用范围
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    ELISA
    特异性
    Human IgG (Fc) Antibody, mAb, Mouse recognizes human IgG and human Fc-tagged proteins.
    产品特性
    Human IgG (Fc) Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human Fc-tagged protein.
    纯化方法
    Protein A affinity column
    免疫原
    Human Fc-tagged protein
    克隆位点
    27B11E9
    亚型
    IgG1 kappa
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0. 05-0.1 µg/mL
    Western Blot: 1 µg/mL
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
    浓度
    0.5 mg/mL
    缓冲液
    lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Human IgG (Fc) Antibody, mAb, Mouse is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    Human IgG is the major immunoglobulin in blood and plays a key role in the humoral immune response. The Fc portion of human IgG binds to Fc receptor that can activate the complement system. Fc-tagged proteins are generated with DNA recombinant technology. The protein of interest is fused with the Fc tag. Most vectors encoding for Fc tag sequence use the Fc region of human IgG1. Protein A can be used for the purification of Fc-tagged proteins. Human IgG (Fc), mAb, Mouse is a useful tool for immunoassay of human Fc-tagged proteins and human IgG
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