山羊 anti-兔 Ig Antibody (CypHer5E)
ICC
Polyclonal
CypHer5E
Fab fragment
Quick Overview for 山羊 anti-兔 Ig Antibody (CypHer5E) (ABIN1742571)
抗原
Ig
片段
Fab fragment
克隆类型
多克隆
标记
CypHer5E
应用范围
Immunocytochemistry (ICC)
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适用
- 兔
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宿主
- 山羊
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纯化方法
- affinity purified Fab fragments. Rabbit serum albumin was added for stabilization.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Antigen-purified primary antibodies are recommended. Unpurified rabbit serum or total IgG preparations may contain considerable amounts of unspecific IgGs that will bind plenty of the rbFab-CpH resulting in a lower overall labelling efficiency.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light to minimize photo bleeching of the fluorescent dye. Application Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 µg of primary antibody with 5 µg of Fab fragments) in a total volume of 10 µL for 30 min at RT in the dark. Adjust the antibody to the desired dilution and immediately use it for your internalization assay. ICC: image
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缓冲液
- PBS
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注意事项
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
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储存条件
- -20 °C
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储存方法
- Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives.
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- Ig
抗原
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