山羊 anti-小鼠 IgG Antibody (Peroxidase (POD))
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- 抗原 See all IgG products
- IgG
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Peroxidase (POD)
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应用范围
- ELISA, Western Blotting (WB), Dot Blot (DB)
- 特异性
- This peroxidase conjugated antibody is specific for mouse IgG and shows no cross-reactivity with mouse IgA, IgM and other animal species IgG proteins.
- 纯化方法
- This antibody was purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG.
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- 应用备注
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Conjugation: Goat anti-mouse IgG is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK (Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).)
Preparation of Diluent Buffer: Use 0.01M TBS or 0.01M PBS to dilute. See “Recommended Dilutions” below for details.
Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Recommended Dilutions:
- Dot blot (Enhanced chemiluminescent coloration) 1:2000-4000
- WB (DAB coloration) 1:500-3000
- WB (Enhanced chemiluminescent coloration) 1:3000-10000
- Direct ELISA (TMB coloration) 1:20000-40000
- 限制
- 仅限研究用
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- 注意事项
- Do NOT add Sodium Azide!
- 储存条件
- 4 °C
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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