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山羊 anti-小鼠 IgG Antibody (Peroxidase (POD))

ELISA, WB, DB Polyclonal Peroxidase (POD)
产品编号 ABIN1720696
发货至: 中国
  • 抗原 See all IgG products
    IgG
    适用
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    小鼠
    宿主
    • 1760
    • 1547
    • 569
    • 242
    • 112
    • 111
    • 32
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    • 18
    • 15
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    山羊
    克隆类型
    • 3389
    • 175
    • 4
    • 1
    多克隆
    标记
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    Peroxidase (POD)
    应用范围
    • 2037
    • 1985
    • 1839
    • 1786
    • 983
    • 920
    • 896
    • 486
    • 419
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    ELISA, Western Blotting (WB), Dot Blot (DB)
    特异性
    This peroxidase conjugated antibody is specific for mouse IgG and shows no cross-reactivity with mouse IgA, IgM and other animal species IgG proteins.
    纯化方法
    This antibody was purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG.
  • 应用备注

    Conjugation: Goat anti-mouse IgG is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK (Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).)

    Preparation of Diluent Buffer: Use 0.01M TBS or 0.01M PBS to dilute. See “Recommended Dilutions” below for details.

    Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

    Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

    Recommended Dilutions:

    • Dot blot (Enhanced chemiluminescent coloration) 1:2000-4000
    • WB (DAB coloration) 1:500-3000
    • WB (Enhanced chemiluminescent coloration) 1:3000-10000
    • Direct ELISA (TMB coloration) 1:20000-40000

    限制
    仅限研究用
  • 注意事项
    Do NOT add Sodium Azide!
    储存条件
    4 °C
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
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