兔 anti-小鼠 IgG Antibody (PE)
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- 抗原 See all IgG products
- IgG
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- PE
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应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM), Dot Blot (DB)
- Supplier Product No.
- 610-408-c46
- Supplier
- Rockland
- 原理
- Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Phycoerythrin Conjugated
- 交叉反应 （详细）
- This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
- 产品特性
- Anti-Mouse IgG Biotin Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3.
- 纯化方法
- Anti-Mouse IgG subclass pan reactive Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.
- 免疫原
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Immunogen: Anti-Mouse IgG subclass pan reactive Secondary Antibody was produced by repeated immunization with highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
Immunogen Type: Native Protein
- 亚型
- IgG
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM, DB Polyclonal IgG TRITC
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
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- 应用备注
- Application Note: Anti-Mouse IgG subclass pan reactive Phycoerythrin Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:100 - 1:250 IF Microscopy Dilution: 1:100 - 1:250 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 0.5 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. IgG1, IgG2a, IgG2b and IgG3 chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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