驴 anti-小鸡 IgY Antibody (Agarose Beads)
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北京 101111
Quick Overview for 驴 anti-小鸡 IgY Antibody (Agarose Beads) (ABIN1381671)
抗原
标记
应用范围
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适用
- 小鸡
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宿主
- 驴
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纯化方法
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Antibodies were isolated from immune sera using affinity chromatography and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.
The specific activity of the anti-IgY-agarose was determined by applying 2.7 mg IgY in 24 mL PBS (0.1 mg/mL) to 1.0 mL bed volume of agarose. Two mL aliquots were collected. The total amount of IgY that bound to the column was calculated. For this lot, 1.3 mg IgY bound per mL agarose. -
免疫原
- A donkey was immunized with highly purified chicken IgY.
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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限制
- 仅限研究用
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状态
- Gel
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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- IgY
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物质类
- Antibody
抗原
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