Quick Overview for 山羊 anti-小鼠 IgG, IgM Antibody (HRP) - Preadsorbed (ABIN129742)
抗原
IgG, IgM
克隆类型
多克隆
标记
HRP
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
亚型
IgG
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适用
- 小鼠
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宿主
- 山羊
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Supplier Product No.
- 610-103-115
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Supplier
- Rockland
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原理
- Mouse IgG + IgM (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against to Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against No reaction was observed against bovine, horse or human serum proteins.
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产品特性
- Anti-Mouse IgG3 Peroxidase Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain).
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纯化方法
- This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
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Immunogen: Anti-Mouse IgG and IgM whole molecule was produced by repeated immunization with Mouse IgG and IgM whole molecule in goat.
Immunogen Type: Native Protein
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应用备注
- Application Note: Anti-Mouse IgG and IgM Peroxidase conjugated antibody has been tested by ELISA and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:40,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG, IgM
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别名
- IgG,IgM
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物质类
- Antibody
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背景
- Anti-Mouse IgG and IgM whole molecule peroxidase conjugated antibody generated in goat detects specifically Mouse IgG and IgM whole molecule. This secondary peroxidase conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
抗原
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