山羊 anti-小鼠 IgG, IgM Antibody (FITC) - Preadsorbed
WB, DB, FM
Polyclonal
IgG
FITC
Quick Overview for 山羊 anti-小鼠 IgG, IgM Antibody (FITC) - Preadsorbed (ABIN129741)
抗原
IgG, IgM
克隆类型
多克隆
标记
FITC
应用范围
Western Blotting (WB), Dot Blot (DB), Fluorescence Microscopy (FM)
亚型
IgG
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适用
- 小鼠
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宿主
- 山羊
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Supplier Product No.
- 610-102-115
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Supplier
- Rockland
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原理
- Mouse IgG + IgM (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against to Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins.
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产品特性
- Anti-Mouse IgG3 Fluorescein Antibody generated in goat detects reactivity to Mouse IgG3 (Gamma 3 chain).
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纯化方法
- This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Optional[Immunogen]: Mouse IgG and IgM whole molecules
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Goat anti-Mouse IgG, IgM antibody (HRP) - Preadsorbed
ELISA, WB, IHC Polyclonal IgG HRP
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应用备注
- Application Note: Anti-Mouse IgG + IgM Fluorescein Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. IF Microscopy Dilution: 1:500 - 1:2,500 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 2.2 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG, IgM
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别名
- IgG,IgM
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物质类
- Antibody
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背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG + IgM Fluorescein Antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
抗原
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