小鼠 anti-小鸡 IgY Antibody - Preadsorbed
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北京 101111
Quick Overview for 小鼠 anti-小鸡 IgY Antibody - Preadsorbed (ABIN126817)
抗原
克隆类型
应用范围
克隆位点
亚型
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适用
- 小鸡
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宿主
- 小鼠
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纯化方法
- Preadsorption: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
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免疫原
- Chicken egg yolk immunoglobulin (IgY) Molecular weight is 150 kDa
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应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunoprecipitation: 1 - 10 g.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mL2xPBS, 0.09 % Sodium Azide, PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C/-80 °C
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储存方法
- Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 30 °C. Thawed aliquots may be stored at 0 °C up to 3 months.
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- IgY
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别名
- IgG + IgY
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背景
- Synonyms: Chicken Immunoglobulin G, Chicken Immunoglobulin Y
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分子量
- 150 kDa
抗原
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