小鼠 anti-人 Ig (Chain lambda), (Light Chain) Antibody (FITC)
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北京 101111
Quick Overview for 小鼠 anti-人 Ig (Chain lambda), (Light Chain) Antibody (FITC) (ABIN118381)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
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抗原表位
- Chain lambda, Light Chain
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适用
- 人
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宿主
- 小鼠
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纯化方法
- Affinity chromatography on Protein A
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免疫原
- Purified human IgG. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
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Goat anti-Human Ig (Chain kappa) antibody (Biotin)
ELISA, FACS, IHC, WB Polyclonal IgG Biotin
Goat anti-Human Ig antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human Ig antibody (FITC)ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Human Ig antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Human Ig antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human Ig (Chain lambda) antibodyELISA, FACS, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human Ig (Chain kappa) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Human Ig (Chain lambda) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
Goat anti-Human Ig (Chain lambda) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
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应用备注
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Flow cytometry: use neat antibody to label 10^6 cells or 10 μL washed whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Ig
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别名
- Immunoglobulin
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背景
- All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5 kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L, Lambda, 0.4-4.2 mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
抗原
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