山羊 anti-人 Ig (Chain lambda), (Light Chain) Antibody (Biotin)
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北京 101111
Quick Overview for 山羊 anti-人 Ig (Chain lambda), (Light Chain) Antibody (Biotin) (ABIN117720)
抗原
片段
克隆类型
标记
应用范围
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抗原表位
- Chain lambda, Light Chain
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适用
- 人
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宿主
- 山羊
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产品特性
- Molar Ratio: 10-20 BAC molecules per Goat IgG Fab molecule.
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纯化方法
- Immunoaffinity chromatography.
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免疫原
- Human lambda (lambda) light chain
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Goat anti-Human Ig (Chain kappa) antibody (Biotin)
ELISA, FACS, IHC, WB Polyclonal IgG Biotin
Goat anti-Human Ig antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human Ig antibody (FITC)ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Human Ig antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Human Ig antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human Ig (Chain lambda) antibodyELISA, FACS, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human Ig (Chain lambda) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Human Ig (Chain kappa) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
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应用备注
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Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring extremelylow background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titerand specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 20,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Ig
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别名
- Immunoglobulin
抗原
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