兔 anti-马 IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB, DB
Polyclonal
Alkaline Phosphatase (AP)
产品编号 ABIN117211
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 兔 anti-马 IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP)) (ABIN117211)
抗原
IgG
克隆类型
多克隆
标记
Alkaline Phosphatase (AP)
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
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抗原表位
- Whole Molecule
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适用
- 马
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宿主
- 兔
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纯化方法
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Immunoaffinity chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads. -
免疫原
- Horse IgG whole molecule.
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Goat anti-Horse IgG (F(ab')2 Region) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry aswell as other phosphatase-antibody based enzymatic assays requiring lot-to-lotconsistency. This product has been assayed against 1.0 μg of Horse IgG in a standardcapture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at roomtemperature. A working dilution of 1:6,000 to 1:30,000 of the reconstitution concentrationis suggested for this product.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0, with 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. -
储存条件
- 4 °C
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- IgG
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物质类
- Antibody
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背景
- Synonyms: Horse IgG, Horse Immunoglobulin G
抗原
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