兔 anti-Cow IgG (Whole Molecule) Antibody (Biotin) - Preadsorbed
WB, IF, IHC (fro), EIA
Polyclonal
Biotin
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- 抗原 See all IgG products
- IgG
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抗原表位
- Whole Molecule
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 产品特性
- Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
- Bovine IgG whole molecule
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- 应用备注
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ImmunoblottingELISAImmunohistochemistryImmunomicroscopyas well as other antibody based assays using streptavidin or avidin conjugates requiringlot-to-lot consistency. This product has been assayed against 1.0 μg of Bovine IgG in a standard capture ELISAusing Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 2.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) sodium azide as preservative. N-Hydroxysuccinimide Ester (BAC).
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Dilute only prior to immediate use
- 储存条件
- 4 °C
- 储存方法
- Store vial at 2-8 °C short term only.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Synonyms: Bovine Immunoglobulin G
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