小鼠 anti-人 IgA Antibody
IHC (fro), IHC (p)
Monoclonal
DA43D3
IgG1
unconjugated
产品编号 ABIN111961
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 小鼠 anti-人 IgA Antibody (ABIN111961)
抗原
IgA
克隆类型
单克隆
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
DA43D3
亚型
IgG1
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适用
- 人
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宿主
- 小鼠
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纯化方法
- Affinity Chromatography
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Mouse anti-Human IgA (Heavy Chain) antibody
ELISA, WB, IHC (fro), IP Monoclonal Mc24-2E11 IgG1 unconjugated
Mouse anti-Human IgA antibodyELISA, IHC (p) Monoclonal AD2-947 IgG1 unconjugated
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应用备注
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Suitable for Immunohistochemistry on Frozen (1 μg/mL, 1/400) and Paraffin EmbeddedSections. Proteinase K pretreatment for antigen retrieval is recommended. Suggested Positive Control: Human tonsil.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
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Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature
1. Wash in PBS
2. Block endogenous peroxidase
3. Wash in PBS4. Block with 10 % normal goat serum in PBS for 30 min. in a humid chamber
5. Incubate with primary antibody (dilution see datasheet) for 1 h in a humid chamber
6. Wash in PBS
7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1 h in a humid chamber
8. Wash in PBS
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS
11. Counterstain with Mayer's hemalum. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- PBS, pH 7.2 with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- Biohazard Informations: This product contains thimerosal which is hazardous.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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- IgA
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物质类
- Antibody
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背景
- Synonyms: Human Immunoglobulin A
抗原
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