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山羊 anti-小鼠 IgG2a Antibody (Biotin)

Name: 山羊 anti-小鼠 IgG2a Antibody (Biotin)
SKU: ABIN1118481
Availability: OutOfStock

Rockland 610-106-041 ELISA, IHC, WB Polyclonal IgG Biotin

(1 reference)

产品编号 ABIN1118481

Supplier Product No.: 610-106-041

发货至: 中国

Datasheet as PDF

抗原 See all IgG2a products

IgG2a
适用
97

21

1
小鼠
宿主
55

28

16

15

5
山羊
克隆类型
69

31
多克隆
标记
23

13

13

12

9

5

5

5

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Biotin
应用范围
61

46

40

34

24

19

17

10

10

8

4

4

3

3

2

2

2

1

1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.

610-106-041

Supplier

Rockland

原理

Mouse IgG2a Antibody Biotin Conjugated Pre-adsorbed

交叉反应（详细）

Minimal crossreactivity against to Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.

产品特性

Anti-Mouse IgG1 Biotin Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain).

纯化方法

MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Immunogen: Mouse IgG2a heavy chain
Immunogen Type: Native Protein

亚型

IgG
Goat anti-Mouse IgG2a (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Rat anti-Mouse IgG2a antibody
ELISA, FACS Monoclonal SB84a IgG1 unconjugated

Rat anti-Mouse IgG2a antibody (HRP)
ELISA, IHC Monoclonal SB84a IgG1 HRP

Rat anti-Mouse IgG2a antibody (PE)
ELISA, FACS Monoclonal SB84a IgG1 PE

Goat anti-Mouse IgG2a (Heavy Chain) antibody (TRITC)
ELISA, FACS, IHC Polyclonal IgG TRITC

Goat anti-Mouse IgG2a antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE

Goat anti-Mouse IgG2a antibody (APC) - Preadsorbed
ELISA, FACS Polyclonal IgG APC

Goat anti-Mouse IgG2a antibody (Alkaline Phosphatase (AP))
ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Mouse IgG2a antibody
ELISA, FLISA Polyclonal IgG unconjugated

Goat anti-Mouse IgG2a (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)

应用备注

Application Note: Mouse IgG2a Biotin Antibody has been tested by ELISA and is available in a variety of formats. Anti-Mouse IgG2a biotin conjugated secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2a antibody based assays. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store second antibody at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Jia, Xu, Smeets, Buhl, Moeller, Floege, Klinkhammer, Boor: "The Role of Platelet-Derived Growth Factor in Focal Segmental Glomerulosclerosis." in: Journal of the American Society of Nephrology : JASN, Vol. 34, Issue 2, pp. 241-257, (2023) (PubMed).
抗原

IgG2a

Abstract

IgG2a 产品

物质类

Antibody

背景

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.