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Quick Overview for 山羊 anti-小鼠 IgG2a Antibody (HRP) (ABIN1118479)
抗原
克隆类型
标记
应用范围
亚型
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适用
- 小鼠
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宿主
- 山羊
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Supplier Product No.
- 610-103-041
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Supplier
- Rockland
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原理
- Mouse IgG2a Antibody Peroxidase Conjugated Pre-adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.
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产品特性
- Anti-Mouse IgG1 Peroxidase Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain).
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免疫原
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Immunogen: Mouse IgG2a heavy chain
Immunogen Type: Native Protein
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Goat anti-Mouse IgG2a (Heavy Chain) antibody (HRP)
ELISA, WB, IHC Polyclonal IgG HRP
Rat anti-Mouse IgG2a antibodyELISA, FACS Monoclonal SB84a IgG1 unconjugated
Rat anti-Mouse IgG2a antibody (HRP)ELISA, IHC Monoclonal SB84a IgG1 HRP
Rat anti-Mouse IgG2a antibody (PE)ELISA, FACS Monoclonal SB84a IgG1 PE
Goat anti-Mouse IgG2a (Heavy Chain) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Mouse IgG2a antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Goat anti-Mouse IgG2a antibody (APC) - PreadsorbedELISA, FACS Polyclonal IgG APC
Rat anti-Mouse IgG2a antibody (Alkaline Phosphatase (AP))ELISA, IHC Monoclonal SB84a IgG1 Alkaline Phosphatase (AP)
Goat anti-Mouse IgG2a (Heavy Chain) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
Goat anti-Mouse IgG2a antibody - PreadsorbedELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
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应用备注
- Application Note: Anti-Mouse IgG2a Peroxidase antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2a antibody based assays. Mouse IgG2a secondary antibody is available in a variety of formats. HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG2a
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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