山羊 anti-小鼠 IgG1 Antibody (Alkaline Phosphatase (AP)) - Preadsorbed
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北京 101111
Quick Overview for 山羊 anti-小鼠 IgG1 Antibody (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN1118472)
抗原
克隆类型
标记
应用范围
亚型
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适用
- 小鼠
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宿主
- 山羊
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Supplier Product No.
- 610-105-040
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Supplier
- Rockland
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原理
- Mouse IgG1 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.
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产品特性
- Anti-Mouse IgG IgA IgM Peroxidase Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM.
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纯化方法
- Anti-Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Mouse IgG1 heavy chain
Immunogen Type: Native Protein
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Goat anti-Mouse IgG1 (Heavy Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgG1 (Heavy Chain) antibodyELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
Rat anti-Mouse IgG1 antibodyELISA, FACS Monoclonal SB77e IgG2b unconjugated
Goat anti-Mouse IgG1 antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Goat anti-Mouse IgG1 antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Mouse IgG1 (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
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应用备注
- Application Note: Anti-Mouse IgG1 Alkaline Phosphatase Conjugated Secondary antibody has been tested by ELISA and dot blot and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:10,000 - 1:40,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.1 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. Conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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- IgG1
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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