山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (DyLight 633)
IA
Polyclonal
DyLight 633
Quick Overview for 山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody (DyLight 633) (ABIN1110731)
抗原
IgG
克隆类型
多克隆
标记
DyLight 633
应用范围
Immunoassay (IA)
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 山羊
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特异性
- Based on immunoelectrophoresis, this antibody reacts with: (gamma) chains on rat IgG light chains on all rat immunoglobulins.
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产品特性
- Goat anti-Rat IgG (H&L) - DyLight® 633 Conjugated is a secondary antibody conjugated to DyLight® 633, which binds to rat IgG (H&L) in immunological assays. DyLight® 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).
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纯化方法
- Affinity purification
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免疫原
- Purified rat IgG, whole molecule
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应用备注
- 1:20 - 1:2,000 for most applications
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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缓冲液
- 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v).
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注意事项
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Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. -
储存条件
- 4 °C/-20 °C
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储存方法
- Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- IgG
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物质类
- Antibody
抗原
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