山羊 anti-大鼠 IgG2a Antibody (FITC)
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- 抗原 See all IgG2a products
- IgG2a
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- 产品特性
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subclass specific
Physicochemical characteristics: IgG protein concentration 10 mg/mL. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.7.
Physical form: Fluorochrome-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Fluorescent marker: Fluorescein isothiocyanate isomer 1. Excitation: 492 nm, emission: 515 nm.
Conjugation procedure: A proprietary technique for the binding to FITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. - 纯化方法
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Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt- precipitation and purification of the IgG fraction by DEAE-chromatography.
Adsorption: Undesired traces of antibody activity are eliminated by immunoaffinity chromatography as required. - 免疫原
- Pools of highly purified homogenous IgG2a isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Mouse anti-Rat IgG2a antibody
ELISA, FACS Monoclonal 2A8F4 IgG1 unconjugated
Mouse anti-Rat IgG2a antibody (Biotin)ELISA, FACS Monoclonal 2A8F4 IgG1 Biotin
Mouse anti-Rat IgG2a antibody (PE)ELISA, FACS Monoclonal 2A8F4 IgG1 PE
Mouse anti-Rat IgG2a antibody (FITC)ELISA, FACS Monoclonal 2A8F4 IgG1 FITC
Mouse anti-Rat IgG2a antibody (Alkaline Phosphatase (AP))ELISA, IHC Monoclonal 2A 8F4 IgG1 Alkaline Phosphatase (AP)
Mouse anti-Rat IgG2a antibody (HRP)ELISA, IHC Monoclonal 2A 8F4 IgG1 HRP
Mouse anti-Rat IgG2a antibody (Cy5)ELISA, FACS Monoclonal 2A8F4 IgG1 Cy5
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- 应用备注
- Working dilutions are usually between 1:20 and 1:80.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
- 缓冲液
- Phosphate buffered saline (PBS, pH 7.2)
- 储存液
- Without preservative
- 注意事项
- Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +0 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- 抗原
- IgG2a
- Abstract
- IgG2a 产品
- 物质类
- Antibody
- 背景
- To identify and measure IgG2a, antigen or antibody, at the cellular and subcellular level by immuno- fluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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