小鼠 anti-人 IgAc, IgA1, IgA2 (Secretory Component) Antibody
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北京 101111
Quick Overview for 小鼠 anti-人 IgAc, IgA1, IgA2 (Secretory Component) Antibody (ABIN1099693)
抗原
克隆类型
应用范围
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抗原表位
- Secretory Component
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适用
- 人
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宿主
- 小鼠
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产品特性
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IgAc, IgA1, IgA2 SC (Class Specific)T
he reactivity of the antibody is restricted to the given protein as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins.
Monoclonal antibody concentration in the solution is 1.0 mg/mL.
Physical form: Delipidated, heat inactivated, lyophilized stable ascites. -
纯化方法
- Ascites
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免疫原
- Highly purified monoclonal IgA subclasses isolated from pooled human serum, secretory components isolated from human milk.
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应用备注
- The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly form 1:100 to 1:500, in ELISA from 1:1000 upwards, in Western blotting 1:2,000 to 1:10,000. These data should be interpreted as general recommendations only.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.
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缓冲液
- No foreign proteins added.
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注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation. This will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgAc, IgA1, IgA2
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背景
- To identify the presence of IgA and secretory subclasses in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgA, subclasses or secretory component and immunoblotting.
抗原
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