山羊 anti-人 Ig (Free & Bound) Antibody - Preadsorbed
IEP, ODD
Polyclonal
unconjugated
产品编号 ABIN1099686
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 山羊 anti-人 Ig (Free & Bound) Antibody - Preadsorbed (ABIN1099686)
抗原
Ig
克隆类型
多克隆
应用范围
Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD)
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抗原表位
- Free & Bound
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适用
- 人
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宿主
- 山羊
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原理
- Goat anti Human Ig kappa light chain (free and bound)
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特异性
- Precipitating polyclonal Goat antiSerum to free and bound Human Ig kappa light chain
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasma. To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immunodiffusion.
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纯化方法
- Delipidated, heat inactivated lyophilized whole serum.
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免疫原
- A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- Precipitation assays. In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.
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缓冲液
- Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Ig
抗原
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