兔 anti-犬 IgG (Heavy & Light Chain) Antibody (Biotin)
ELISA, IHC, WB
Polyclonal
IgG
Biotin
F(ab')2 fragment
1 image
Quick Overview for 兔 anti-犬 IgG (Heavy & Light Chain) Antibody (Biotin) (ABIN1044014)
抗原
IgG
片段
F(ab')2 fragment
克隆类型
多克隆
标记
Biotin
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
亚型
IgG
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抗原表位
- Heavy & Light Chain
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适用
- 犬
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宿主
- 兔
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Supplier Product No.
- 704-406-002
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Supplier
- Rockland
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原理
- F(ab')2 Dog IgG (H&L) Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Dog IgG and Dog Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
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产品特性
- F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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免疫原
- Optional[Immunogen]: Dog IgG whole molecule
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Mouse anti-Dog IgG antibody
Animal-Free ELISA Monoclonal AB169-1-E12 IgG2a Recombinant Antibody unconjugated
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应用备注
- Application Note: F(ab')2 Anti-Dog IgG Biotin Antibody has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000-1:5,000 Western Blot Dilution: 1:2,000-1:10,000 ELISA Dilution: 1:20,000 - 1:100,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
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浓度
- 1 mg/mL
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缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
抗原
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