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Quick Overview for 山羊 anti-小鼠 IgG2b Antibody (ABIN1043979)
抗原
克隆类型
应用范围
亚型
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适用
- 小鼠
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宿主
- 山羊
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Supplier Product No.
- 610-101-042
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Supplier
- Rockland
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原理
- Mouse IgG2b Antibody Pre-adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against to Bv, Hu & Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG2b. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA for human and rabbit. For IgG1, IgG2a and IgG3 reactivity was less than 5 % against target (IgG2b, IgG). Cross-reactivity against Bovine is assessed only by IEP.
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产品特性
- Anti-Mouse IgG2a Antibody generated in goat detects reactivity to Mouse IgG2a (Gamma 2a chain).
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纯化方法
- Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2b coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Mouse IgG2b heavy chain
Immunogen Type: Native Protein
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Rabbit anti-Mouse IgG2b antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgG2b (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgG2b (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgG2b (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG2b antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Rat anti-Mouse IgG2b antibody (FITC)ELISA, FACS, FLISA Monoclonal SB74g IgG2b FITC
Goat anti-Mouse IgG2b antibody (Biotin) - PreadsorbedELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Mouse IgG2b antibody (FITC) - PreadsorbedELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Mouse IgG2b antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgG2b (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
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应用备注
- Application Note: Mouse IgG2b secondary antibody has been tested by dot blot and ELISA and is available in a variety of formats. Anti-Mouse IgG2b secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays. Immunohistochemistry Dilution: 1:2,000 - 1:10,000 Western Blot Dilution: 1:4,000 - 1:20,000 ELISA Dilution: 1:40,000 - 1:200,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Preservative:0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IgG2b
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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