山羊 anti-小鼠 IgG1 Antibody
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- 抗原 See all IgG1 products
- IgG1
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)
- Supplier Product No.
- 610-101-040
- Supplier
- Rockland
- 原理
- Mouse IgG1 Antibody Pre-adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against to Bv Hu & Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA for human and rabbit. For IgG2a, IgG2b and IgG3 reactivity was less than 5 % against target (IgG1, IgG). Cross-reactivity against Bovine is assessed only by IEP.
- 产品特性
- Anti-Mouse IgG Antibody generated in goat detects reactivity to Mouse IgG.
- 纯化方法
- Anti-Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Mouse IgG1 heavy chain
Immunogen Type: Native Protein
- 亚型
- IgG
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Goat anti-Mouse IgG1 (Heavy Chain) antibody (HRP)
ELISA, WB, IHC Polyclonal IgG HRP
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
Rat anti-Mouse IgG1 antibodyELISA, FACS Monoclonal SB77e IgG2b unconjugated
Rat anti-Mouse IgG1 antibody (HRP)ELISA Monoclonal SB77E IgG2b HRP
Goat anti-Mouse IgG1 (Heavy Chain) antibodyELISA, WB, IHC, FACS Polyclonal IgG unconjugated
Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgG1 (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)ELISA, WB, IHC, FACS Polyclonal IgG Biotin
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
Goat anti-Mouse IgG1 antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE F(ab')2 fragment
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- 应用备注
- Application Note: Anti-Mouse IgG1 secondary antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays. Immunohistochemistry Dilution: 1:2,000 - 1:10,000 Western Blot Dilution: 1:4,000 - 1:20,000 ELISA Dilution: 1:40,000 - 1:200,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.2 mg/mL
- 缓冲液
- Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Preservative:0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- IgG1
- Abstract
- IgG1 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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