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山羊 anti-人 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG HRP

Western Blot of Goat anti-Human IgG Pre-Adsorbed Peroxidase Conjugated Secondary Antibody. (山羊 anti-人 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed)

1 image

(1 reference)

产品编号 ABIN1043965

Supplier Product No.: 609-1316-0100

发货至: 中国

Quick Overview for 山羊 anti-人 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed (ABIN1043965)

抗原

IgG

克隆类型

多克隆

标记

HRP

应用范围

ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

亚型

IgG

抗原表位
2396

517

417

146

130

128

66

29

28

28

21

9

5

5

2

2

2

2

2

1

1

1
Heavy & Light Chain
适用
752

703

607

468

410

244

213

176

146

141

124

110

107

101

82

62

47

28

27

16

15

10

8

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
人
宿主
1772

1583

567

282

122

112

41

32

22

21

16

11

11
山羊

Supplier Product No.

609-1316-0100

Supplier

Rockland

原理

Human IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

交叉反应（详细）

Minimal crossreactivity against MOUSE Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.

产品特性

Anti-Human IgG (gamma chain) Peroxidase generated in goat detects human Immunoglobulin G (gamma chain).

纯化方法

Human IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

免疫原

Immunogen: Human IgG whole molecule
Immunogen Type: Native Protein
Mouse anti-Human IgG (Fc Region) antibody
ELISA, WB, FACS, ICC Monoclonal EM-07 IgG1 unconjugated

Mouse anti-Human IgG (Fc Region) antibody
Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody unconjugated

Mouse anti-Human IgG (Fc Region) antibody (HRP)
ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP

Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG1 kappa unconjugated

Mouse anti-Human IgG (Fc Region) antibody (HRP)
ELISA, WB Monoclonal EM-07 IgG1 HRP

Mouse anti-Human IgG (Fc Region) antibody (HRP)
Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP

Goat anti-Human IgG antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC

Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Human IgG (Heavy & Light Chain) antibody
ELISA, IHC, WB Polyclonal IgG unconjugated

应用备注

Application Note: Anti-Human IgG (H&L) Peroxidase Conjugate has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:400,000 - 1:600,000 Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

储存液

Gentamicin sulfate

注意事项

This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Muñoz, Beltrán-Alzate, Duthie, Serrano-Coll, Cardona-Castro: "Comparison of Enzyme-Linked Immunosorbent Assay Using Either Natural Octyl Disaccharide-Leprosy IDRI Diagnostic or Phenolic Glycolipid-I Antigens for the Detection of Leprosy Patients in Colombia." in: The American journal of tropical medicine and hygiene, Vol. 98, Issue 1, pp. 274-277, (2018) (PubMed).
抗原

IgG

物质类

Antibody

背景

It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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