驴 anti-豚鼠 IgG (Heavy & Light Chain) Antibody - Preadsorbed
ELISA, WB
Polyclonal
IgG
unconjugated
1 image
Quick Overview for 驴 anti-豚鼠 IgG (Heavy & Light Chain) Antibody - Preadsorbed (ABIN1043951)
抗原
IgG
克隆类型
多克隆
应用范围
ELISA, Western Blotting (WB)
亚型
IgG
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抗原表位
- Heavy & Light Chain
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适用
- 豚鼠
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宿主
- 驴
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Supplier Product No.
- 606-701-c36
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Supplier
- Rockland
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原理
- Guinea Pig IgG (H&L) Antibody Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Bv Ch Gt Ham Hs Hu Ms Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Guinea Pig IgG and with light chains of most Guinea Pig immunoglobulins.
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产品特性
- Anti-Guinea Pig IgG Rhodamine Antibody generated in donkey detects guinea pig IgG.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Guinea Pig IgG whole molecule
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Goat anti-Guinea Pig IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG HRP
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应用备注
- Application Note: Anti-Guinea Pig IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:25,000 - 1:75,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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