兔 anti-小鼠 IgM Antibody (FITC)
Quick Overview for 兔 anti-小鼠 IgM Antibody (FITC) (ABIN102685)
抗原
克隆类型
标记
应用范围
亚型
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适用
- 小鼠
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宿主
- 兔
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Supplier Product No.
- 610-4207
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Supplier
- Rockland
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原理
- Mouse IgM (mu chain) Antibody Fluorescein Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgM and Mouse Serum. No reaction was observed against other mouse heavy or light chain proteins.
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产品特性
- Anti-Mouse IgG F(ab')2 Fluorescein antibody generated in rabbit recognizes the dimeric Fab portion of the mouse IgG molecule. Mouse IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind mouse IgG F(c) will not bind mouse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Optional[Immunogen]: Mouse IgM whole molecule
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Goat anti-Mouse IgM (Heavy Chain) antibody (TRITC)
ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Mouse IgM (Heavy Chain) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
Goat anti-Mouse IgM (Heavy Chain) antibody (Biotin)ELISA, FACS, IHC, WB Polyclonal IgG Biotin
Goat anti-Mouse IgM antibody (APC) - PreadsorbedELISA, FACS Polyclonal IgG APC
Goat anti-Mouse IgM antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Goat anti-Mouse IgM (Heavy Chain) antibody (Cy5)FACS, IHC Polyclonal IgG Cy5
Rat anti-Mouse IgM antibody (PE)ELISA, FACS Monoclonal 1B4B1 IgG1 PE
Rat anti-Mouse IgM antibody (PE-Cy7)ELISA, FACS Monoclonal 1B4B1 IgG1 PE-Cy7
Goat anti-Mouse IgM antibody (PE-Cy7) - PreadsorbedELISA, FACS Polyclonal IgG PE-Cy7
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应用备注
- Application Note: Anti-Mouse IgM antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.5 mg/mL
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缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgM
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物质类
- Antibody
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背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM Fluorescein antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
抗原
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