山羊 anti-猴 IgM Antibody (Texas Red (TR))
FACS, FLISA, FM
Polyclonal
IgG
Texas Red (TR)
Quick Overview for 山羊 anti-猴 IgM Antibody (Texas Red (TR)) (ABIN102653)
抗原
IgM
克隆类型
多克隆
标记
Texas Red (TR)
应用范围
Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
亚型
IgG
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适用
- 猴
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宿主
- 山羊
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Supplier Product No.
- 617-109-007
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Supplier
- Rockland
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原理
- Monkey IgM (mu chain) Antibody Texas Red™ Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Monkey IgM and Monkey Serum. No reaction was observed against other monkey heavy or light chain proteins.
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产品特性
- Anti-Monkey IgG (gamma chain) biotin conjugated antibody generated in goat detects specifically Monkey IgG (gamma chain). This anti-Monkey biotin conjugated secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Optional[Immunogen]: Monkey IgM whole molecule
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Goat anti-Monkey IgM antibody (FITC)
FACS, WB, DB, FLISA, FM Polyclonal IgG FITC
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应用备注
- Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgM
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物质类
- Antibody
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背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Monkey IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
抗原
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