Quick Overview for 山羊 anti-猴 IgM Antibody (HRP) (ABIN102650)
抗原
IgM
克隆类型
多克隆
标记
HRP
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)
亚型
IgG
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适用
- 猴
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宿主
- 山羊
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Supplier Product No.
- 617-103-007
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Supplier
- Rockland
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原理
- Monkey IgM mu Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Monkey IgM and Monkey Serum. No reaction was observed against other Monkey heavy or light chain proteins.
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产品特性
- Anti-Monkey IgG (gamma chain) generated in goat detects monkey Immunoglobulin G (gamma chain).
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
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Immunogen: Monkey IgM whole molecule
Immunogen Type: Native Protein
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Goat anti-Monkey IgM antibody (Biotin)
ELISA, IHC, WB Polyclonal IgG Biotin
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应用备注
- Application Note: Anti-Monkey IgM (mu chain) peroxidase conjugated antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500-1:2,500 Western Blot Dilution: 1:1,000-1:5,000 ELISA Dilution: 1:65,000 - 1:160,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgM
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物质类
- Antibody
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背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Monkey IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
抗原
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