兔 anti-犬 IgM Antibody
ELISA, IHC, WB
Polyclonal
unconjugated
Quick Overview for 兔 anti-犬 IgM Antibody (ABIN102570)
抗原
IgM
克隆类型
多克隆
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
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适用
- 犬
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宿主
- 兔
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Supplier Product No.
- 104-4107
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Supplier
- Rockland
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原理
- Dog IgM mu chain Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Dog IgM and Dog Serum. No reaction was observed against Dog IgG.
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纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Optional[Immunogen]: Dog IgM mu heavy chain
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Rabbit anti-Dog IgM antibody (HRP)
ELISA, IHC, WB, DB Polyclonal IgG HRP
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应用备注
- Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
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浓度
- 60 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgM
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物质类
- Antibody
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背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Dog IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
抗原
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