Quick Overview for 山羊 anti-小鸡 IgM Antibody (ABIN102563)
抗原
克隆类型
应用范围
亚型
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适用
- 小鸡
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宿主
- 山羊
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Supplier Product No.
- 603-101-007
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Supplier
- Rockland
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原理
- Chicken IgM (mu chain) Secondary Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgM and Chicken Serum. No reaction was observed against other Chicken heavy or light chain proteins.
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产品特性
- Anti-Chicken IgG Antibody generated in goat detects chicken IgY.
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纯化方法
- Chicken Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Chicken IgM whole molecule
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Mouse anti-Chicken IgM antibody (PE)
ELISA, FACS Monoclonal M-1 IgG2b PE
Mouse anti-Chicken IgM antibody (SPRD)ELISA, FACS Monoclonal M-1 IgG2b SPRD
Mouse anti-Chicken IgM antibody (Biotin)ELISA, FACS Monoclonal M-1 IgG2b Biotin
Mouse anti-Chicken IgM antibody (APC)ELISA, FACS, FLISA Monoclonal M-1 IgG2b APC
Mouse anti-Chicken IgM antibody (FITC)ELISA, FACS Monoclonal M-1 IgG2b FITC
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应用备注
- Application Note: Anti-Chicken IgM antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Anti-IgM secondary antibody at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IgM
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物质类
- Antibody
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背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Chicken IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
抗原
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