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Quick Overview for 兔 anti-小鼠 IgG3 Antibody (HRP) (ABIN102437)
抗原
克隆类型
标记
应用范围
亚型
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适用
- 小鼠
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宿主
- 兔
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Supplier Product No.
- 610-4343
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Supplier
- Rockland
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原理
- Mouse IgG3 (Gamma 3 chain) Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other mouse or human heavy or light chain isotypes.
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产品特性
- Anti-Mouse IgG2b peroxidase conjugated antibody generated in rabbit detects specifically mouse IgG2b. This secondary peroxidase conjugated antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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纯化方法
- Anti-MOUSE IgG3 (Gamma 3 chain) (RABBIT) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
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Immunogen: Anti-Mouse IgG3 heavy chain was produced by repeated immunization with Mouse IgG3 heavy chain in rabbit.
Immunogen Type: Native Protein
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Rat anti-Mouse IgG3 antibody (Biotin)
ELISA, FACS, FLISA Monoclonal SB76b IgG2a Biotin
Goat anti-Mouse IgG3 antibody (FITC) - PreadsorbedELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Mouse IgG3 antibody (HRP) - PreadsorbedELISA Polyclonal IgG HRP
Goat anti-Mouse IgG3 (Heavy Chain) antibody (HRP)ELISA, WB, IHC Polyclonal IgG HRP
Goat anti-Mouse IgG3 (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
Rat anti-Mouse IgG3 antibodyELISA, FACS, FLISA Monoclonal SB76b IgG2a unconjugated
Goat anti-Mouse IgG3 (Heavy Chain) antibodyELISA, WB, IHC, FACS Polyclonal IgG unconjugated
Goat anti-Mouse IgG3 antibody - PreadsorbedELISA Polyclonal IgG unconjugated
Rat anti-Mouse IgG3 antibody (HRP)ELISA, FACS, FLISA Monoclonal SB76b IgG2a HRP
Rat anti-Mouse IgG3 antibody (FITC)ELISA, FACS, FLISA Monoclonal SB76b IgG2a FITC
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应用备注
- Application Note: Mouse IgG3 heavy chain Peroxidase Conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:75,000 - 1:125,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG3
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物质类
- Antibody
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背景
- Anti-Mouse IgG3 heavy chain antibody generated in rabbit detects specifically Mouse IgG3 heavy chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
抗原
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