兔 anti-小鼠 IgG2b Antibody (TRITC)
Quick Overview for 兔 anti-小鼠 IgG2b Antibody (TRITC) (ABIN102406)
抗原
克隆类型
标记
应用范围
亚型
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适用
- 小鼠
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宿主
- 兔
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Supplier Product No.
- 610-4042
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Supplier
- Rockland
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原理
- Mouse IgG2b (Gamma 2b chain) Antibody Rhodamine Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse Serum. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other mouse heavy or light chain isotypes.
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产品特性
- Anti-Mouse IgG2a Rhodamine Antibody generated in rabbit detects reactivity to Mouse IgG2a (Gamma 2a chain).
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Optional[Immunogen]: Mouse IgG2b heavy chain
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Goat anti-Mouse IgG2b (Heavy Chain) antibody (FITC)
ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgG2b (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG2b antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Rat anti-Mouse IgG2b antibody (FITC)ELISA, FACS, FLISA Monoclonal SB74g IgG2b FITC
Goat anti-Mouse IgG2b antibody (Biotin) - PreadsorbedELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Mouse IgG2b (Heavy Chain) antibody (Texas Red (TR))ELISA, IHC, FACS Polyclonal IgG Texas Red (TR)
Goat anti-Mouse IgG2b antibody (FITC) - PreadsorbedELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Rat anti-Mouse IgG2b antibody (PE)ELISA, FACS, FLISA Monoclonal SB74g IgG2b PE
Goat anti-Mouse IgG2b antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE F(ab')2 fragment
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应用备注
- Application Note: Anti-Mouse IgG2b Rhodamine Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG2b
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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