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Quick Overview for 兔 anti-小鼠 IgG2b Antibody (ABIN102397)
抗原
克隆类型
应用范围
亚型
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适用
- 小鼠
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宿主
- 兔
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Supplier Product No.
- 610-4142
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Supplier
- Rockland
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原理
- Mouse IgG2b (Gamma 2b chain) Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum, Mouse IgG and Mouse IgG2b. Specificity was confirmed by ELISA. For IgG1, IgG2a and IgG3 reactivity was less than 5 % against target (IgG2b, IgG).
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产品特性
- Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse IgG2a heavy chain. Anti-Mouse IgG2a is ideal for investigators involved in Serum Protein Component research.
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纯化方法
- Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2b coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Mouse IgG2b heavy chain
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Rabbit anti-Mouse IgG2b antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgG2b (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgG2b (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgG2b (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG2b antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Rat anti-Mouse IgG2b antibody (FITC)ELISA, FACS, FLISA Monoclonal SB74g IgG2b FITC
Goat anti-Mouse IgG2b antibody (Biotin) - PreadsorbedELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Mouse IgG2b antibody (FITC) - PreadsorbedELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Mouse IgG2b antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgG2b (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
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应用备注
- Application Note: Anti-Mouse IgG2b antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IgG2b
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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