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兔 anti-小鼠 IgG2a Antibody

ELISA, IHC, WB, DB Polyclonal IgG unconjugated
Rockland
产品编号 ABIN102373
Supplier Product No.: 610-4141
发货至: 中国

Quick Overview for 兔 anti-小鼠 IgG2a Antibody (ABIN102373)

抗原

IgG2a

克隆类型

  • 70
  • 32
多克隆

应用范围

  • 59
  • 44
  • 44
  • 38
  • 23
  • 19
  • 16
  • 12
  • 10
  • 10
  • 5
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

亚型

IgG
  • 适用

    • 99
    • 21
    • 1
    小鼠

    宿主

    • 54
    • 30
    • 17
    • 15
    • 5

    Supplier Product No.

    610-4141

    Supplier

    Rockland

    原理

    Mouse IgG2a Antibody

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum, Mouse IgG and Mouse IgG2a. Specificity was confirmed by ELISA. For IgG1, IgG2b and IgG3 reactivity was less than 5 % against target (IgG2a, IgG).

    产品特性

    Anti-Mouse IgG1 Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain).

    纯化方法

    Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    过滤

    Sterile filtered

    免疫原

    Optional[Immunogen]: Mouse IgG2a heavy chain
  • 应用备注

    Application Note: Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:2,000 - 1:10,000 Western Blot Dilution: 1:4,000 - 1:20,000 ELISA Dilution: 1:15,000 - 1:30,000 Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.02 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

    有效期

    12 months
  • 抗原

    IgG2a

    物质类

    Antibody

    背景

    Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse IgG2a heavy chain. Anti-Mouse IgG2a is ideal for investigators involved in Serum Protein Component research.
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