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Quick Overview for 兔 anti-小鼠 IgG1 Antibody (HRP) (ABIN102337)
抗原
克隆类型
标记
应用范围
亚型
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适用
- 小鼠
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宿主
- 兔
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Supplier Product No.
- 610-4340
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Supplier
- Rockland
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原理
- Mouse IgG1 Secondary Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA against other mouse or human heavy chain isotypes. Cross reactivity with Mouse IgG2b and IgG3 is 1 % .
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产品特性
- Anti-Mouse IgG F(ab')2 Peroxidase Antibody generated in rabbit recognizes the dimeric Fab portion of the mouse IgG molecule. Mouse IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind mouse IgG F(c) will not bind mouse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Optional[Immunogen]: Mouse IgG1 heavy chain
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Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgG1 (Heavy Chain) antibody (HRP)ELISA, WB, IHC Polyclonal IgG HRP
Rat anti-Mouse IgG1 antibodyELISA, FACS Monoclonal SB77e IgG2b unconjugated
Goat anti-Mouse IgG1 (Heavy Chain) antibodyELISA, WB, IHC, FACS Polyclonal IgG unconjugated
Rat anti-Mouse IgG1 antibody (HRP)ELISA Monoclonal SB77E IgG2b HRP
Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Goat anti-Mouse IgG1 (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)ELISA, WB, IHC, FACS Polyclonal IgG Biotin
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
Goat anti-Mouse IgG1 antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE F(ab')2 fragment
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应用备注
- Application Note: Mouse IgG1 secondary antibody conjugated to HRP is available in a variety of formats. Anti-Mouse IgG1 Peroxidase Antibody has been tested by ELISA and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:20,000 - 1:200,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG1
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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