兔 anti-绵羊 IgG (Fc Region) Antibody (Alkaline Phosphatase (AP))
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Quick Overview for 兔 anti-绵羊 IgG (Fc Region) Antibody (Alkaline Phosphatase (AP)) (ABIN102287)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Fc Region
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适用
- 绵羊
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宿主
- 兔
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Supplier Product No.
- 613-4503
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Supplier
- Rockland
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原理
- Sheep IgG Fc Antibody Alkaline Phosphatase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab).
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产品特性
- Anti-Sheep IgG antibody generated in rabbit detects specifically sheep IgG. This secondary antibody anti-Sheep is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Sheep IgG F(c) fragment
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Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB, DB Polyclonal IgG Biotin
Donkey anti-Sheep IgG antibody (DyLight 680) - PreadsorbedWB, FLISA, FM Polyclonal IgG DyLight 680
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应用备注
- Application Note: Anti-Sheep IgG F(c) Alkaline Phosphatase has been tested by ELISA and dot blot and is assayed against 1.0 μg of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,300 to 1:5,400 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Receptors bind the Fc portion of sheep IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
抗原
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