兔 anti-绵羊 IgG (F(ab')2 Region) Antibody (HRP) - Preadsorbed
Quick Overview for 兔 anti-绵羊 IgG (F(ab')2 Region) Antibody (HRP) - Preadsorbed (ABIN102283)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- F(ab')2 Region
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适用
- 绵羊
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宿主
- 兔
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Supplier Product No.
- 613-4330
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Supplier
- Rockland
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原理
- Sheep IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab')2 and Sheep Serum. No reaction was observed against Sheep IgG F(c) or Human Serum Proteins.
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产品特性
- Anti-Sheep IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Optional[Immunogen]: Sheep IgG F(ab')2 fragment
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Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Donkey anti-Sheep IgG antibody (DyLight 680) - PreadsorbedWB, FLISA, FM Polyclonal IgG DyLight 680
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB, DB Polyclonal IgG Biotin
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应用备注
- Application Note: Anti-Sheep IgG F(ab')2 antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Anti-Sheep IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the sheep IgG molecule. Sheep IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind sheep IgG F(c) will not bind sheep IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG F(ab')2 is conjugated to Peroxidase.
抗原
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