兔 anti-绵羊 IgG (F(ab')2 Region) Antibody - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- F(ab')2 Region
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适用
- 绵羊
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- IgG F(ab')2
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: immunoaffinity chromatography using Sheep IgG coupled to agarose beads
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Sheep IgG F(ab')2 fragment
- 亚型
- IgG
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Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (HRP)
ELISA, WB Polyclonal IgG HRP
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB, LF Polyclonal IgG unconjugated
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (HRP)ELISA, WB Polyclonal IgG HRP
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Sheep IgG F(ab')2 antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Rabbit Anti Sheep IgG F(ab')2 Antibody, Rabbit Anti-Sheep IgG Fab2 Antibody, Rabbit Anti-Sheep IgG Fab2 Fragment Antibody
Background: Anti-Sheep IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the sheep IgG molecule. Sheep IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind sheep IgG F(c) will not bind sheep IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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