兔 anti-绵羊 IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed
Quick Overview for 兔 anti-绵羊 IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed (ABIN102260)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 绵羊
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宿主
- 兔
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Supplier Product No.
- 613-4628
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Supplier
- Rockland
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原理
- Sheep IgG (H&L) Secondary Antibody Biotin Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.
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产品特性
- Anti-Sheep IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the sheep IgG molecule. Sheep IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind sheep IgG F(c) will not bind sheep IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG F(ab')2 is conjugated to Biotin.
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纯化方法
- Secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Optional[Immunogen]: Sheep IgG whole molecule
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Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Donkey anti-Sheep IgG antibody (DyLight 680) - PreadsorbedWB, FLISA, FM Polyclonal IgG DyLight 680
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
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应用备注
- Application Note: Anti-Sheep IgG Biotin Conjugated Antibody has been tested by ELISA and Dot Blot and is suitable for western blot, ELISA and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment. Secondary antibody conjugated to biotin is available in a variety of formats.
抗原
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