驴 anti-绵羊 IgG (Heavy & Light Chain) Antibody
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北京 101111
Quick Overview for 驴 anti-绵羊 IgG (Heavy & Light Chain) Antibody (ABIN102230)
抗原
克隆类型
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 绵羊
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宿主
- 驴
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Supplier Product No.
- 613-701-168
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Supplier
- Rockland
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原理
- Sheep IgG (H&L) Antibody Pre-adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Ch GP Ham Hs Hu Ms Rb & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
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产品特性
- Anti-Sheep IgG (H&L) Rhodamine Antibody generated in donkey detects reactivity to sheep IgG.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Sheep IgG (H&L) was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Immunogen Type: Native Protein
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Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Donkey anti-Sheep IgG antibody (DyLight 680) - PreadsorbedWB, FLISA, FM Polyclonal IgG DyLight 680
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB, DB Polyclonal IgG Biotin
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应用备注
- Application Note: Anti-Sheep IgG (H&L) has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:39,200 - 1:59,200 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Anti-Sheep IgG antibody generated in donkey detects specifically sheep IgG. This secondary antibody anti-Sheep is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
抗原
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