兔 anti-大鼠 IgG (Heavy & Light Chain) Antibody (HRP)
(1 reference)
Quick Overview for 兔 anti-大鼠 IgG (Heavy & Light Chain) Antibody (HRP) (ABIN102159)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 兔
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Supplier Product No.
- 612-4302
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Supplier
- Rockland
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原理
- Rat IgG (H&L) Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG and Rat Serum.
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产品特性
- Anti-Rat IgG (H&L) Fluorescein Antibody generated in rabbit detects reactivity to Rat IgG.
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纯化方法
- Secondary Antibody Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Optional[Immunogen]: Rat IgG whole molecule
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Goat anti-Rat IgG (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
Rabbit anti-Mouse IgG antibody (HRP) - PreadsorbedELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment
Goat anti-Rat IgG antibody (DyLight 488)WB, FLISA, FM Polyclonal IgG DyLight 488
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG Atto 647N
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rat IgG antibody (Cy3) - PreadsorbedWB, FACS, FLISA, FM, DB Polyclonal IgG Cy3
Goat anti-Rat IgG antibodyELISA Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Rat IgG (Heavy Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
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应用备注
- Application Note: Anti-Rat IgG Peroxidase conjugated antibody has been tested by ELISA and is suitable for western blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:170,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 2.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "KDM5 lysine demethylases are involved in maintenance of 3'UTR length." in: Science advances, Vol. 2, Issue 11, pp. e1501662, (2017) (PubMed).
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: "KDM5 lysine demethylases are involved in maintenance of 3'UTR length." in: Science advances, Vol. 2, Issue 11, pp. e1501662, (2017) (PubMed).
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- IgG
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物质类
- Antibody
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背景
- When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.
抗原
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