山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody - Preadsorbed
Quick Overview for 山羊 anti-大鼠 IgG (Heavy & Light Chain) Antibody - Preadsorbed (ABIN102136)
抗原
克隆类型
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 山羊
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Supplier Product No.
- 612-101-120
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Supplier
- Rockland
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原理
- Rat IgG (H&L) Antibody Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
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产品特性
- Anti-Rat IgG (H&L) generated in goat detects rat Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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纯化方法
- Anti-RAT IgG (H&L) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Rat IgG whole molecule
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Goat anti-Rat IgG (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
Rabbit anti-Mouse IgG antibody (HRP) - PreadsorbedELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment
Goat anti-Rat IgG antibody (DyLight 488)WB, FLISA, FM Polyclonal IgG DyLight 488
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG Atto 647N
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rat IgG antibody (Cy3) - PreadsorbedWB, FACS, FLISA, FM, DB Polyclonal IgG Cy3
Goat anti-Rat IgG antibodyELISA Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Rat IgG (Heavy Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
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应用备注
- Application Note: Anti-Rat IgG antibody has been tested by ELISA and western blot and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Specific conditions should be optimized by user. Immunohistochemistry Dilution: 1:500 - 1:2,000 Western Blot Dilution: 1:1,000 - 1:4,000 ELISA Dilution: 1:10,000 - 1:40,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- RAT IgG (H&L) (GOAT) Antibody was generated in goat detects specifically Rat IgG whole molecule. Anti-Rat IgG antibody is ideal for investigators involved in serum protein component research.
抗原
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