ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Supplier Product No.
211-7103
Supplier
Rockland
原理
Rabbit IgG Fc Antibody
交叉反应 (详细)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab).
产品特性
Anti-Rabbit IgG (H&L) Fluorescein Antibody generated in mouse detects reactivity to Rabbit IgG.
纯化方法
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Application Note: Anti-Rabbit IgG F(c) antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 5.0 mL
缓冲液
Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Preservative:0.01 % (w/v) Sodium Azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.