山羊 anti-兔 IgG (F(ab')2 Region) Antibody (HRP) - Preadsorbed
Quick Overview for 山羊 anti-兔 IgG (F(ab')2 Region) Antibody (HRP) - Preadsorbed (ABIN102053)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- F(ab')2 Region
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适用
- 兔
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宿主
- 山羊
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Supplier Product No.
- 611-1324
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Supplier
- Rockland
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原理
- Rabbit IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed
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交叉反应 （详细）
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab')2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c) or Human Serum Proteins.
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产品特性
- Anti-Rabbit IgG F(c) peroxidase antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
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纯化方法
- Anti-RABBIT IgG F(ab')2 (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.
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免疫原
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Immunogen: Anti-Rabbit IgG F(ab')2 fragment was produced by repeated immunization with Rabbit IgG F(ab')2 fragment in goat.
Immunogen Type: Native Protein
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应用备注
- Application Note: Anti-Rabbit IgG F(ab')2 fragment has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:10,000 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:130,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 2.0 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Anti-Rabbit IgG F(ab')2 fragment antibody generated in goat detects specifically Rabbit IgG F(ab')2 fragment. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
抗原
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