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山羊 anti-兔 IgG (F(ab')2 Region) Antibody

ELISA, IHC, WB Polyclonal unconjugated
Rockland
产品编号 ABIN102038
Supplier Product No.: 111-1104
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for 山羊 anti-兔 IgG (F(ab')2 Region) Antibody (ABIN102038)

抗原

IgG

克隆类型

  • 3429
  • 166
  • 12
  • 1
多克隆

应用范围

  • 1760
  • 1691
  • 1684
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  • 353
  • 232
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  • 1
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  • 1
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  • 1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
  • 抗原表位

    • 2348
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    • 311
    • 133
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    • 67
    • 65
    • 29
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    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    F(ab')2 Region

    适用

    • 658
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    • 580
    • 402
    • 374
    • 236
    • 194
    • 155
    • 128
    • 126
    • 115
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    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1

    宿主

    • 1575
    • 1459
    • 550
    • 214
    • 97
    • 84
    • 38
    • 25
    • 21
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    • 16
    • 12
    • 11
    山羊

    Supplier Product No.

    111-1104

    Supplier

    Rockland

    原理

    Rabbit IgG F(ab')2 Antibody

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Rabbit IgG, Rabbit IgG F(ab')2 and Rabbit Serum.  No reaction was observed against Rabbit IgG F(c).

    产品特性

    Anti-Rabbit IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

    纯化方法

    This product was prepared from monospecific antiserum by a delipidation and defibrination.

    免疫原

    Optional[Immunogen]: Rabbit IgG F(ab')2 fragment
  • 应用备注

    Application Note: Anti-Rabbit IgG F(ab')2 antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

    浓度

    31.0 mg/mL

    缓冲液

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:None

    储存液

    Without preservative

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    有效期

    12 months
  • 抗原

    IgG

    物质类

    Antibody

    背景

    Anti-Rabbit IgG F(ab')2 Antibody generated in goat recognizes the dimeric Fab portion of the rabbit IgG molecule. Rabbit IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind rabbit IgG F(c) will not bind rabbit IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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