绵羊 anti-兔 IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))
Quick Overview for 绵羊 anti-兔 IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN102021)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 兔
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宿主
- 绵羊
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Supplier Product No.
- 611-6502
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Supplier
- Rockland
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原理
- Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum, Rabbit IgG and Rabbit Serum.
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产品特性
- Anti-Rabbit IgG peroxidase conjugated antibody generated in sheep detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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纯化方法
- Anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Rabbit IgG whole molecule
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应用备注
- Application Note: Anti-Rabbit IgG Alkaline Phosphatase antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry applications. Specific conditions for reactivity should be optimized by the researcher. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:15,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- Rabbit IgG antibody detects rabbit IgG whole molecule heavy and light chain components. Anti-Rabbit IgG antibody is ideal for researchers using immunoassays with rabbit primary antibody reagents.
抗原
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