小鸡 anti-兔 IgG (Heavy & Light Chain) Antibody (HRP)
(1 reference)
Quick Overview for 小鸡 anti-兔 IgG (Heavy & Light Chain) Antibody (HRP) (ABIN101939)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Heavy & Light Chain
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适用
- 兔
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宿主
- 小鸡
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Supplier Product No.
- 611-903-002
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Supplier
- Rockland
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原理
- Rabbit IgG (H&L) Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Chicken Serum, Rabbit IgG and Rabbit Serum.
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产品特性
- Anti-Rabbit IgG (H&L) Fluorescein Antibody generated in chicken detects reactivity to Rabbit IgG.
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose.
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免疫原
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Immunogen: Anti-Rabbit IgG whole molecule was produced by repeated immunization with Rabbit IgG whole molecule in chicken.
Immunogen Type: Native Protein
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Mouse anti-Rabbit IgG antibody (HRP)Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP
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Goat anti-Rabbit IgG antibody (DyLight 488)WB, IF, FLISA, FM, DB, MA Polyclonal IgG DyLight 488
Goat anti-Rabbit IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
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应用备注
- Application Note: Anti-Rabbit IgG Peroxidase Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:140,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "α- and β-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage." in: Experimental and therapeutic medicine, Vol. 13, Issue 3, pp. 1143-1150, (2017) (PubMed).
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: "α- and β-Naphthoflavone synergistically attenuate H2O2-induced neuron SH-SY5Y cell damage." in: Experimental and therapeutic medicine, Vol. 13, Issue 3, pp. 1143-1150, (2017) (PubMed).
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- IgG
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物质类
- Antibody
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背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
抗原
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